Research Article

Identifying interaction partners of the Arabidopsis thaliana deubiquitinase‐associated WD40‐repeat proteins LRS1 and WDR20

Published: 2013-4

Journal: The FASEB Journal

DOI: 10.1096/fasebj.27.1_supplement.785.6

Abstract

The lateral root stimulator 1 (LRS1) protein ofArabidopsis thalianacontains a WD40‐repeat domain similar to those found in proteins involved in protein trafficking, ubiquitin modification, and proteasome targeting. The human homolog of LRS1,ubiquitin‐specific proteaseassociatedfactor ‐1 (UAF1), binds theubiquitin–specificproteases USP1, USP12, and USP46. USP1 is activated by UAF1 binding, which is required to regulate the Fanconi anemia DNA damage repair pathway. InC. elegans,USP‐46 regulates glutamate receptor abundance by targeting it for lysosomal degradation. Co‐immunoprecipitations have shown that LRS1 forms a complex with theA. thalianahomolog of WDR20 andubiquitinbindingprotein 3 (UBP3), a USP1/12/46 homolog. To elucidate targets of the LRS1/UBP3/WDR20 complex we screened a yeast two‐hybrid expression library with LRS1 as bait. We identified ten potential interacting partners, including a zinc finger RING/ FYVE/PHD‐ domain containing protein, a leucine‐rich repeat containing protein, and a kinase involved in hormone signaling. The interaction strengths were analyzed by plating serial dilutions on selective media and β‐galactosidase assays. Ongoing experiments include expression library screening with WDR20 and UBP3 to identify additional interaction partners. This research was supported by Guerrieri and Henson Undergraduate Research programs.

Faculty Members

  • Patti T. Erickson - Biological Sciences Salisbury University Salisbury MD
  • Sean Ryan James - Biological Sciences Salisbury University Salisbury MD
  • Floyd L. Erickson - Biological Sciences Salisbury University Salisbury MD

Themes

  • Ubiquitin modification
  • DNA damage repair
  • Hormone signaling
  • Lateral root development
  • Protein complex formation
  • Protein interactions

Categories

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