Cloning and expression of loop 1 and loop 2 adiponectin receptor one mutants
Abstract
Adiponectin (Acrp 30) is a 30 kDa protein hormone that is released into the blood stream by adipocytes and is partly responsible for controlling fatty acid oxidation and glucose metabolism. Low levels of Acrp 30 can lead to the onset of obesity associated diseases such as type 2 diabetes. Adiponectin binds to a seven transmembrane receptor, Adiponectin Receptor 1 (Adipo R1) to influence cellular energy levels. Understanding how Acrp 30 binds to and influences cellular energy levels may lead to new targets for the treatment of obesity and diabetes. To begin this determination, the importance of loop 1 and loop 2 of AdipoR1 in the physiologically relevant binding of Acrp 30 will be assessed. Loop 1 and 2 mutants were produced using Overlap Extension PCR (OEPCR). The mutated sequence was based on the corresponding region within the protein PAQR 4. This protein is a member of the same family as AdipoR1, yet it does not bind Acrp 30. Once verified by sequencing, expression of the mutant proteins and Acrp 30 downstream signaling will be analyzed to determine if either loop region is critical in protein stability and/or downstream signaling events. If either region is determined to play a role in binding by analysis of downstream signaling, future researchers could develop new medical treatments that can be used to reduce not only the risks but also the incidence of obesity. Currently, Loop 1 and 2 of Adipo R1 have been produced and are undergoing sequence analysis to ensure that the desired mutation is in place. This work is supported by the Henson School of Science and Technology and Salisbury University.
Faculty Members
- Chasta L Parker - Chemistry Salisbury University Salisbury MD
- Allison Nicole Ose - Chemistry Salisbury University Salisbury MD
- Dana Jones - Chemistry Salisbury University Salisbury MD
Themes
- Role of adiponectin in metabolic regulation
- Potential therapeutic targets for obesity-related diseases
- Mechanisms of adiponectin receptor signaling
- Influence of adiponectin on obesity and diabetes
Categories
- Human medical genetics
- Oncology and cancer biology
- Biochemistry
- Health sciences, other
- Public health, general
- Molecular biology
- Biochemistry, biophysics, and molecular biology
- Molecular genetics
- Medical clinical science
- Health services research
- Health sciences, general
- Biochemistry and molecular biology
- Genetics and genomics
- Physiology, oncology and cancer biology
- Health sciences
- Physiology, oncology and cancer biology nec
- Biological and biomedical sciences
- Medical, biomedical, and health informatics
- Public health