Research Article

Effect of adiponectin on human colorectal‐cancer cell proliferation

Published: 2011-4

Journal: The FASEB Journal

DOI: 10.1096/fasebj.25.1_supplement.919.1

Abstract

Adiponectin (Acrp30) is a protein hormone released from adipocytes that has been shown to have an anti‐proliferative effect on cancer cells. Three cellular receptors from the progestin and AdipoQ Receptor (PAQR) family have been identified that can actively bind Acrp30: AdipoR1 and AdipoR2, located on the cell membrane; and AdipoR3 in the Golgi body. Currently, it is known that AdipoR1 and R2 allow Acrp30 to regulate fatty acid and glucose metabolism as well as cell growth and proliferation. Prior research has indicated that AdipoR1 and AdipoR2 may be involved in the Acrp30 anti‐proliferative effect but there has been minimal research on if or how AdipoR3 (PAQR3) may be involved. To determine a possible relationship between Acrp30, AdipoR3, and cell proliferation, the production of mRNA in HT‐29 human colo‐rectal cancer cells in the presence or absence of Acrp30 was quantified using quantitative real‐time PCR (qRT‐PCR). These mRNA levels were also compared to the levels of AdipoR1 and R2. Briefly, the mRNA of the cell was extracted, converted to cDNA, and evaluated by qRT‐PCR using fluorescent probe primers. Following this study, RNAi knockdown experiments of AdipoR3 will be performed after which the anti‐proliferative effect of Acrp30 on HT‐29 cells will be reanalyzed. Preliminary results confirm the anti‐proliferative nature of Acrp30 and also indicate altered AdipoR3 mRNA abundance in adiponectin treated colo‐rectal cancer cells. This work was supported by the Henson School of Science and Technology, Salisbury University, and the Cort Scholarship.

Faculty Members

  • Chasta L Parker - Chemistry Salisbury University Salisbury MD
  • Matthew James Copeland - Chemistry Salisbury University Salisbury MD
  • Velora A Branch - Chemistry Salisbury University Salisbury MD

Themes

  • Effects of hormone signaling on cell proliferation
  • Role of adiponectin in cancer biology
  • Interactions between receptors and cancer cell behavior
  • Molecular mechanisms of anti-cancer properties

Categories

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